NEET Zoology Biotechnology - Principles and Processes PYQ Analysis 2020–2026: Topic-wise Trends & Practice
Biotechnology - Principles and Processes (NEET Zoology): 46 questions in 2020–2026, 11.9% of the subject. Topic-wise trends, difficulty split, repeated topics and PYQ practice.
Edurack Team
October 13, 2026
Biotechnology - Principles and Processes gave 46 of the 388 NEET Zoology questions asked between 2020 and 2026 (Re-NEET 2026 included). That is 11.9% of the subject and the #1 chapter out of 16 by weightage, about 5.8 questions per paper. It peaked at 8 in NEET 2021.
Of the 46 questions tagged for difficulty, 37% were easy, 46% medium and 17% hard, so this is a fairly balanced mix of easy and moderate questions.
Its weight has stayed steady: 6.7 questions per paper in 2020–2022 and 5 across 2025, 2026 and Re-NEET 2026.
Year-wise questions from Biotechnology - Principles and Processes
| Paper | Questions |
|---|---|
| NEET 2020 | 5 |
| NEET 2021 | 8 |
| NEET 2022 | 7 |
| NEET 2023 | 6 |
| NEET 2024 | 5 |
| NEET 2025 | 6 |
| NEET 2026 | 5 |
| Re-NEET 2026 | 4 |
Difficulty split
| Level | Questions | Share |
|---|---|---|
| Easy | 17 | 37% |
| Medium | 21 | 46% |
| Hard | 8 | 17% |
Topic-wise analysis
Every topic tagged under Biotechnology - Principles and Processes, most-asked first. "Papers" is the number of the 8 papers in which the topic appeared.
| Topic | Total Qs | Easy | Medium | Hard | Papers | Asked in |
|---|---|---|---|---|---|---|
| Polymerase Chain Reaction (Denaturation, Annealing, etc.) | 7 | 3 | 3 | 1 | 5 | 2021, 2022, 2025, 2026 and Re-NEET 2026 |
| Gel Electrophoresis (Separation and Isolation of DNA Fragments) | 5 | 2 | 2 | 1 | 5 | 2020, 2021, 2022, 2023 and 2025 |
| Cloning Vectors (Plasmid (pBR322)) | 5 | 1 | 3 | 1 | 4 | 2020, 2022, 2024 and Re-NEET 2026 |
| Cloning Vectors (pBR322, Cosmids, etc.)u– | 4 | 2 | 1 | 1 | 3 | 2023, 2025 and 2026 |
| Processes of Recombinant DNA Technologies (Isolation of the Genetic Material (DNA)) | 4 | 1 | 1 | 2 | 3 | 2021, 2023 and Re-NEET 2026 |
| Restriction Enzymes (Endonuclease and Exonuclease, Nomenclature of Restr…) | 4 | 2 | 2 | 0 | 3 | 2020, 2024 and 2026 |
| Tools of Recombinant DNA Technology (Restriction Enzymes and Other Enzymes) | 4 | 1 | 3 | 0 | 3 | 2020, 2021 and 2022 |
| Identification of Clones having Recombinant DNA (Antibiotic Resistance Method) | 2 | 0 | 1 | 1 | 2 | 2021 and 2022 |
| Identification of Clones having Recombinant DNA (Blue White r– Screening Method (Insertional Inactivati…) | 2 | 1 | 1 | 0 | 2 | 2022 and 2025 |
| Obtaining the Foreign Gene Product (Bioreactors) | 2 | 2 | 0 | 0 | 2 | 2024 and 2025 |
| Principles of Biotechnology (Introduction) | 2 | 1 | 1 | 0 | 2 | 2022 and 2023 |
| Cloning Vectors (Vectors for Plants and Animals (Ti Plasmid, Retrovi…) | 1 | 1 | 0 | 0 | 1 | 2024 |
| Methods of Gene Transfer (Gene Gun, Electroporation, etc.) | 1 | 0 | 1 | 0 | 1 | 2023 |
| Processes of Recombinant DNA Technologies (Agarose Gel Electrophoresis) | 1 | 0 | 1 | 0 | 1 | 2026 |
| Processes of Recombinant DNA Technologies (Amplification of eGene of Interest Using PCR) | 1 | 0 | 1 | 0 | 1 | 2025 |
| Processes of Recombinant DNA Technologies (Insertion of Recombinant DNA into the Host Cell/Org…) | 1 | 0 | 0 | 1 | 1 | 2024 |
Which topics matter most
Most-tested topics
- Polymerase Chain Reaction (Denaturation, Annealing, etc.): 7 Qs
- Gel Electrophoresis (Separation and Isolation of DNA Fragments): 5 Qs
- Cloning Vectors (Plasmid (pBR322)): 5 Qs
- Cloning Vectors (pBR322, Cosmids, etc.)u–: 4 Qs
- Processes of Recombinant DNA Technologies (Isolation of the Genetic Material (DNA)): 4 Qs
Topics that keep coming back
These appeared in three or more different papers:
- Polymerase Chain Reaction (Denaturation, Annealing, etc.): 5 papers (2021, 2022, 2025, 2026 and Re-NEET 2026)
- Gel Electrophoresis (Separation and Isolation of DNA Fragments): 5 papers (2020, 2021, 2022, 2023 and 2025)
- Cloning Vectors (Plasmid (pBR322)): 4 papers (2020, 2022, 2024 and Re-NEET 2026)
- Cloning Vectors (pBR322, Cosmids, etc.)u–: 3 papers (2023, 2025 and 2026)
- Processes of Recombinant DNA Technologies (Isolation of the Genetic Material (DNA)): 3 papers (2021, 2023 and Re-NEET 2026)
- Restriction Enzymes (Endonuclease and Exonuclease, Nomenclature of Restr…): 3 papers (2020, 2024 and 2026)
- Tools of Recombinant DNA Technology (Restriction Enzymes and Other Enzymes): 3 papers (2020, 2021 and 2022)
What the latest papers asked
Topics from NEET 2026 and Re-NEET 2026:
- Polymerase Chain Reaction (Denaturation, Annealing, etc.)
- Cloning Vectors (Plasmid (pBR322))
- Cloning Vectors (pBR322, Cosmids, etc.)u–
- Processes of Recombinant DNA Technologies (Isolation of the Genetic Material (DNA))
- Restriction Enzymes (Endonuclease and Exonuclease, Nomenclature of Restr…)
- Processes of Recombinant DNA Technologies (Agarose Gel Electrophoresis)
Where the hard questions came from
- Processes of Recombinant DNA Technologies (Isolation of the Genetic Material (DNA)): 2 hard of 4
- Polymerase Chain Reaction (Denaturation, Annealing, etc.): 1 hard of 7
- Cloning Vectors (Plasmid (pBR322)): 1 hard of 5
- Gel Electrophoresis (Separation and Isolation of DNA Fragments): 1 hard of 5
- Cloning Vectors (pBR322, Cosmids, etc.)u–: 1 hard of 4
- Identification of Clones having Recombinant DNA (Antibiotic Resistance Method): 1 hard of 2
How to use this data
- Start with the 7 recurring topics above. They account for 33 of the chapter's 46 questions (72%).
- Re-solve the 6 topics from NEET 2026 and Re-NEET 2026, since they show the current style of questions.
- Then attempt the chapter's past questions under a timer and note which topics cost you marks.
Practise Biotechnology - Principles and Processes PYQs
Solve every Biotechnology - Principles and Processes question from 2020–2026 with solutions on Edurack:
- Practise Biotechnology - Principles and Processes PYQs
- See the full Biotechnology - Principles and Processes blueprint page
- NEET Zoology PYQ analysis: all chapters
Related chapters
- Animal Kingdom: #2 in Zoology, 38 questions
Frequently Asked Questions
How many questions came from Biotechnology - Principles and Processes in NEET 2020–2026?
Biotechnology - Principles and Processes contributed 46 NEET Zoology questions across the 8 papers from 2020–2026, which is 11.9% of the subject.
Which topics of Biotechnology - Principles and Processes are asked most often?
The most-tested topics are Polymerase Chain Reaction (Denaturation, Annealing, etc.): 7 Qs, Gel Electrophoresis (Separation and Isolation of DNA Fragments): 5 Qs and Cloning Vectors (Plasmid (pBR322)): 5 Qs.
Is Biotechnology - Principles and Processes easy or difficult in NEET?
Of 46 difficulty-tagged questions, 37% were easy, 46% medium and 17% hard.
Was Biotechnology - Principles and Processes asked in NEET 2026?
Yes. It had 5 questions in NEET 2026 and 4 questions in Re-NEET 2026.