Questions
46
across 8 papers
Share of subject
11.9%
of 388 Zoology Qs
Weightage rank
#1
of 16 chapters
Difficulty
17 easy · 21 medium · 8 hard
Biotechnology - Principles and Processes contributed 46 of the 388 NEET Zoology questions between 2020 and 2026 (Re-NEET 2026 included). That is 11.9% of the subject and makes it the #1 chapter out of 16 by weightage — roughly 5.8 questions per paper, peaking at 8 in NEET 2021.
Of the 46 questions tagged for difficulty, 37% were easy, 46% medium and 17% hard — a fairly balanced mix of easy and moderate questions.
Its weight has stayed fairly steady: 5 questions per paper across NEET 2025, NEET 2026 and Re-NEET 2026, against 6.7 in 2020–2022.
The single most-tested topic is Polymerase Chain Reaction (Denaturation, Annealing, etc.) — 7 questions across 5 papers.
16 topics tagged under Biotechnology - Principles and Processes. Numbers show how many questions each topic had in each paper; E / M / H are the easy, medium and hard counts.
| Topic | 2020 | 2021 | 2022 | 2023 | 2024 | 2025 | 2026 | Re | Total | E | M | H |
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Polymerase Chain Reaction (Denaturation, Annealing, etc.) | – | 3 | 1 | – | – | 1 | 1 | 1 | 7 | 3 | 3 | 1 |
| Cloning Vectors (Plasmid (pBR322)) | 1 | – | 1 | – | 1 | – | – | 2 | 5 | 1 | 3 | 1 |
| Gel Electrophoresis (Separation and Isolation of DNA Fragments) | 1 | 1 | 1 | 1 | – | 1 | – | – | 5 | 2 | 2 | 1 |
| Cloning Vectors (pBR322, Cosmids, etc.)u– | – | – | – | 1 | – | 1 | 2 | – | 4 | 2 | 1 | 1 |
| Processes of Recombinant DNA Technologies (Isolation of the Genetic Material (DNA)) | – | 1 | – | 2 | – | – | – | 1 | 4 | 1 | 1 | 2 |
| Restriction Enzymes (Endonuclease and Exonuclease, Nomenclature of Restr…) | 2 | – | – | – | 1 | – | 1 | – | 4 | 2 | 2 | – |
| Tools of Recombinant DNA Technology (Restriction Enzymes and Other Enzymes) | 1 | 2 | 1 | – | – | – | – | – | 4 | 1 | 3 | – |
| Identification of Clones having Recombinant DNA (Antibiotic Resistance Method) | – | 1 | 1 | – | – | – | – | – | 2 | – | 1 | 1 |
| Identification of Clones having Recombinant DNA (Blue White r– Screening Method (Insertional Inactivati…) | – | – | 1 | – | – | 1 | – | – | 2 | 1 | 1 | – |
| Obtaining the Foreign Gene Product (Bioreactors) | – | – | – | – | 1 | 1 | – | – | 2 | 2 | – | – |
| Principles of Biotechnology (Introduction) | – | – | 1 | 1 | – | – | – | – | 2 | 1 | 1 | – |
| Cloning Vectors (Vectors for Plants and Animals (Ti Plasmid, Retrovi…) | – | – | – | – | 1 | – | – | – | 1 | 1 | – | – |
| Methods of Gene Transfer (Gene Gun, Electroporation, etc.) | – | – | – | 1 | – | – | – | – | 1 | – | 1 | – |
| Processes of Recombinant DNA Technologies (Agarose Gel Electrophoresis) | – | – | – | – | – | – | 1 | – | 1 | – | 1 | – |
| Processes of Recombinant DNA Technologies (Amplification of eGene of Interest Using PCR) | – | – | – | – | – | 1 | – | – | 1 | – | 1 | – |
| Processes of Recombinant DNA Technologies (Insertion of Recombinant DNA into the Host Cell/Org…) | – | – | – | – | 1 | – | – | – | 1 | – | – | 1 |
In the NEET papers from 2020 to 2026 (including Re-NEET 2026), 46 Zoology questions came from Biotechnology - Principles and Processes — 11.9% of the subject, or about 5.8 per paper.
By past weightage it ranks #1 of 16 Zoology chapters, so it is one of the five highest-weightage Zoology chapters, with 46 questions over 2020–2026.
The most-tested topics are Polymerase Chain Reaction (Denaturation, Annealing, etc.) (7), Gel Electrophoresis (Separation and Isolation of DNA Fragments) (5) and Cloning Vectors (Plasmid (pBR322)) (5). The topic table above lists every topic with its count in each paper.
Of the 46 difficulty-tagged questions, 37% were easy, 46% medium and 17% hard.
Polymerase Chain Reaction (Denaturation, Annealing, etc.), Cloning Vectors (Plasmid (pBR322)), Cloning Vectors (pBR322, Cosmids, etc.)u– and Processes of Recombinant DNA Technologies (Isolation of the Genetic Material… and more were asked in the 2026 papers.
Edurack has chapter-wise NEET previous-year questions for Biotechnology - Principles and Processes. Use the practice button on this page to open the set, attempt questions and check your answers.
Counts come from Edurack's tagged set of NEET papers 2020–2026 (including Re-NEET 2026), mapped to NCERT chapters and topics. Use them as a trend guide — past frequency doesn't guarantee what a future paper will ask.