NEET Biotechnology and its Applications PYQ practice sheet

16 previous-year NEET Zoology questions from Biotechnology and its Applications, newest papers first. Biotechnology and its Applications carries 27 of the Zoology questions in NEET 2020 to 2026, so this is a good place to test yourself after revising the chapter. Marking: +4 correct, −1 wrong.

1. 1.

   RE-NEET 2026

   The inactive form of Bt toxin is converted to the active form in the insect gut _______

   • (A)
     due to alkaline pH
   • (B)
     by nucleases
   • (C)
     due to acidic pH
   • (D)
     by proteases
2. 2.

   NEET 2026

   The human protein named $\alpha$-1-antitrypsin, obtained from transgenic animals, is used for the treatment of ________.

   • (A)
     Rheumatoid arthritis
   • (B)
     Alzheimer's disease
   • (C)
     Cystic fibrosis
   • (D)
     Emphysema
3. 3.

   NEET 2026

   The toxin proteins isolated from Bacillus thuringiensis, coded by which of the following genes would control cotton bollworms and corn borer, respectively?

   • (A)
     *cry*IAc and *cry*IAb
   • (B)
     *cry*IAc and *cry*IIIAb
   • (C)
     *cry*IIAb and *cry*IAc
   • (D)
     *cry*IAc and *cry*IIAb
4. 4.

   NEET 2024

   Given below are two statements: Statement I: Bt toxins are insect group specific and coded by a gene *cry*IAc. Statement II: Bt toxin exists as inactive protoxin in B. thuringiensis. However, after ingestion by the insect the inactive protoxin gets converted into active form due to acidic pH of the insect gut. In the light of the above statements, choose the correct answer from the options given below:

   • (A)
     Statement I is false but Statement II is true.
   • (B)
     Both Statement I and Statement II are true.
   • (C)
     Both Statement I and Statement II are false.
   • (D)
     Statement I is true but Statement II is false.
5. 5.

   NEET 2024

   Match List I with List II: List I: A. $\alpha$-1 antitrypsin, B. Cry IAb, C. Cry IAc, D. Enzyme replacement therapy List II: I. Cotton bollworm, II. ADA deficiency, III. Emphysema, IV. Corn borer Choose the correct answer from the options given below:

   • (A)
     A-III, B-IV, C-I, D-II
   • (B)
     A-II, B-I, C-IV, D-III
   • (C)
     A-III, B-I, C-II, D-IV
   • (D)
     A-II, B-IV, C-I, D-III
6. 6.

   NEET 2023

   Which one of the following techniques does not serve the purpose of early diagnosis of a disease for its early treatment?

   • (A)
     Enzyme Linked Immuno-Sorbent Assay (ELISA) technique
   • (B)
     Recombinant DNA Technology
   • (C)
     Serum and Urine analysis
   • (D)
     Polymerase Chain Reaction (PCR) technique
7. 7.

   NEET 2022

   In gene therapy for Adenosine Deaminase (ADA) deficiency, the patient requires periodic infusion of genetically engineered lymphocytes because:

   • (A)
     Genetically engineered lymphocytes are not immortal cells.
   • (B)
     Retroviral vector is introduced into these lymphocytes.
   • (C)
     Gene isolated from marrow cells producing ADA is introduced into cells at embryonic stages.
   • (D)
     Lymphocytes from patient's blood are grown in culture, outside the body.
8. 8.

   NEET 2022

   Statements related to human Insulin are given below. Which statement(s) is/are correct about genetically engineered Insulin? (a) Pro-hormone insulin contains extra stretch of C-peptide (b) A-peptide and B-peptide chains of insulin were produced separately in E.coli, extracted and combined by creating disulphide bond between them (c) Insulin used for treating Diabetes was extracted from Cattle and Pigs (d) Pro-hormone Insulin needs to be processed for converting into a mature and functional hormone (e) Some patients develop allergic reactions to the foreign insulin Choose the most appropriate answer from the options given below:

   • (A)
     (c), (d) and (e) only
   • (B)
     (a), (b) and (d) only
   • (C)
     (b) only
   • (D)
     (c) and (d) only
9. 9.

   NEET 2021

   When gene targetting involving gene amplification is attempted in an individual's tissue to treat disease, it is known as :

   • (A)
     Biopiracy
   • (B)
     Molecular diagnosis
   • (C)
     Safety testing
   • (D)
     Gene therapy
10. 10.

    NEET 2021

    Now a days it is possible to detect the mutated gene causing cancer by allowing radioactive probe to hybridise its complimentary DNA in a clone of cells, followed by its detection using autoradiography because :

    • (A)
      mutated gene does not appear on a photographic film as the probe has no complimentarity with it.
    • (B)
      mutated gene does not appear on photographic film as the probe has complimentarity with it.
    • (C)
      mutated gene completely and clearly appears on a photographic film.
    • (D)
      mutated gene partially appears on a photographic film.
11. 11.

    NEET 2021

    With regard to insulin choose correct options. (a) C-peptide is not present in mature insulin. (b) The insulin produced by rDNA technology has C-peptide. (c) The pro-insulin has C-peptide. (d) A-peptide and B-peptide of insulin are interconnected by disulphide bridges. Choose the correct answer from the options given below.

    • (A)
      (b) and (c) only
    • (B)
      (a), (c) and (d) only
    • (C)
      (a) and (d) only
    • (D)
      (b) and (d) only
12. 12.

    NEET 2021

    For effective treatment of the disease, early diagnosis and understanding its pathophysiology is very important. Which of the following molecular diagnostic techniques is very useful for early detection ?

    • (A)
      Southern Blotting Technique
    • (B)
      Hybridization Technique
    • (C)
      ELISA Technique
    • (D)
      Western Blotting Technique
13. 13.

    NEET 2021

    The Adenosine deaminase deficiency results into :

    • (A)
      Dysfunction of Immune system
    • (B)
      Digestive disorder
    • (C)
      Parkinson's disease
    • (D)
      Addison's disease
14. 14.

    NEET 2020

    Bt cotton variety that was developed by the introduction of toxin gene of Bacillus thuringiensis (Bt) is resistant to :

    • (A)
      Insect pests
    • (B)
      Fungal diseases
    • (C)
      Plant nematodes
    • (D)
      Insect predators
15. 15.

    NEET 2020

    Which of the following statements is not correct?

    • (A)
      In man insulin is synthesised as a proinsulin.
    • (B)
      The proinsulin has an extra peptide called C-peptide.
    • (C)
      The functional insulin has A and B chains linked together by hydrogen bonds.
    • (D)
      Genetically engineered insulin is produced in E-Coli.
16. 16.

    NEET 2020

    Match the following columns and select the correct option.

    • (A)
      (a)-(iv), (b)-(i), (c)-(ii), (d)-(iii)
    • (B)
      (a)-(iii), (b)-(ii), (c)-(i), (d)-(iv)
    • (C)
      (a)-(ii), (b)-(iii), (c)-(iv), (d)-(i)
    • (D)
      (a)-(i), (b)-(ii), (c)-(iii), (d)-(iv)