NEET Photosynthesis in Higher Plants PYQ practice sheet

20 previous-year NEET Botany questions from Photosynthesis in Higher Plants, newest papers first. Photosynthesis in Higher Plants carries 22 of the Botany questions in NEET 2020 to 2026, so this is a good place to test yourself after revising the chapter. Marking: +4 correct, −1 wrong.

1. 1.

   RE-NEET 2026

   Photorespiration reaction catalyzed by RuBisCo is shown below: RuBP + $O_2$ $\rightarrow$ 3-Phosphoglycerate + X. Identify "X" from the given options:

   • (A)
     Oxaloacetate
   • (B)
     2-Phosphoglycolate
   • (C)
     Malate
   • (D)
     Phosphoenolpyruvate
2. 2.

   RE-NEET 2026

   Which pigment has absorption peak at 700 nm in the photosynthetic reaction centre PS I (P700)?

   • (A)
     Carotenoids
   • (B)
     Chlorophyll b
   • (C)
     Xanthophylls
   • (D)
     Chlorophyll a
3. 3.

   RE-NEET 2026

   Select the correct sequence of experiments that led to a gradual understanding of photosynthesis in green plants.

   • (A)
     Absorption spectra of chlorophyll a and b $\rightarrow$ production of glucose $\rightarrow$ release of oxygen $\rightarrow$ role of air
   • (B)
     Release of oxygen $\rightarrow$ production of glucose $\rightarrow$ absorption spectra of chlorophyll a and b $\rightarrow$ role of air
   • (C)
     Role of air $\rightarrow$ release of oxygen $\rightarrow$ production of glucose $\rightarrow$ absorption spectra of chlorophyll a and b
   • (D)
     Production of glucose $\rightarrow$ role of air $\rightarrow$ release of oxygen $\rightarrow$ absorption spectra of chlorophyll a and b
4. 4.

   RE-NEET 2026

   How many turns of Calvin cycle are required for the formation of three molecules of glucose?

   • (A)
     6
   • (B)
     1
   • (C)
     3
   • (D)
     18
5. 5.

   RE-NEET 2026

   Which of the following statements regarding photorespiration are correct? (a) Do not occur in C3 plants (b) $CO_2$ is consumed and $O_2$ is generated (c) Phosphoglycolate is formed (d) No synthesis of ATP and NADPH Choose the correct answer from the options given below:

   • (A)
     (b) and (d) only
   • (B)
     (a) and (d) only
   • (C)
     (c) and (d) only
   • (D)
     (a) and (b) only
6. 6.

   NEET 2026

   Find the incorrect statement(s) about photosynthesis from the following: A. The water splitting complex is associated with PS I. B. $\mathrm{C_4}$ plants use the $\mathrm{C_3}$ pathway of $\mathrm{CO_2}$ fixation as the main biosynthetic pathway. C. In $\mathrm{C_4}$ plants, photorespiration does not occur. D. $\mathrm{C_3}$ plants exhibit 'Kranz' anatomy. E. ATP synthesis in chloroplast occurs through chemiosmosis. Choose the answer from the options given below:

   • (A)
     B and C only
   • (B)
     A and D only
   • (C)
     B only
   • (D)
     B and E only
7. 7.

   NEET 2026

   The enzyme required for carboxylation in the Calvin cycle is

   • (A)
     Carboxypeptidase
   • (B)
     PEP carboxylase
   • (C)
     RuBP carboxylase - oxygenase
   • (D)
     Hexokinase
8. 8.

   NEET 2026

   How many ATP and NADPH molecules are required to make one molecule of glucose through the Calvin pathway?

   • (A)
     18 ATP and 12 NADPH
   • (B)
     6 ATP and 12 NADPH
   • (C)
     24 ATP and 18 NADPH
   • (D)
     12 ATP and 18 NADPH
9. 9.

   NEET 2024

   Which of the following are required for the dark reaction of photosynthesis? A. Light B. Chlorophyll C. $\mathrm{CO_2}$ D. ATP E. NADPH Choose the correct answer from the options given below:

   • (A)
     C, D and E only
   • (B)
     A, B and C only
   • (C)
     D and E only
   • (D)
     B, C and D only
10. 10.

    NEET 2024

    How many molecules of ATP and NADPH are required for every molecule of $\mathrm{CO_2}$ fixed in the Calvin cycle?

    • (A)
      2 molecules of ATP and 2 molecules of NADPH
    • (B)
      3 molecules of ATP and 2 molecules of NADPH
    • (C)
      3 molecules of ATP and 3 molecules of NADPH
    • (D)
      2 molecules of ATP and 3 molecules of NADPH
11. 11.

    NEET 2024

    Given below are two statements: Statement I: In $\mathrm{C_3}$ plants, some $\mathrm{O_2}$ binds RuBisCO, hence $\mathrm{CO_2}$ fixation is decreased. Statement II: In $\mathrm{C_4}$ plants, mesophyll cells show very little photorespiration while bundle sheath cells do not show photorespiration. In the light of the above statements, choose the correct answer from the options given below:

    • (A)
      Statement I is false but Statement II is true.
    • (B)
      Both Statement I and Statement II are false.
    • (C)
      Statement I is true but Statement II is false.
    • (D)
      Both Statement I and Statement II are true.
12. 12.

    NEET 2023

    How many ATP and $\mathrm{NADPH_2}$ are required for the synthesis of one molecule of Glucose during Calvin cycle?

    • (A)
      18 ATP and 16 $\mathrm{NADPH_2}$
    • (B)
      12 ATP and 12 $\mathrm{NADPH_2}$
    • (C)
      18 ATP and 12 $\mathrm{NADPH_2}$
    • (D)
      12 ATP and 16 $\mathrm{NADPH_2}$
13. 13.

    NEET 2023

    The reaction centre in PS II has an absorption maxima at

    • (A)
      780 nm
    • (B)
      680 nm
    • (C)
      700 nm
    • (D)
      660 nm
14. 14.

    NEET 2023

    Which of the following combinations is required for chemiosmosis?

    • (A)
      proton pump, electron gradient, NADP synthase
    • (B)
      membrane, proton pump, proton gradient, ATP synthase
    • (C)
      membrane, proton pump, proton gradient, NADP synthase
    • (D)
      proton pump, electron gradient, ATP synthase
15. 15.

    NEET 2022

    Which one of the following is not true regarding the release of energy during ATP synthesis through chemiosmosis? It involves:

    • (A)
      Reduction of NADP to NADPH$_2$ on the stroma side of the membrane
    • (B)
      Breakdown of proton gradient
    • (C)
      Breakdown of electron gradient
    • (D)
      Movement of protons across the membrane to the stroma
16. 16.

    NEET 2022

    Given below are two statements: Statement I: The primary $\mathrm{CO_2}$ acceptor in $C_4$ plants is phosphoenolpyruvate and is found in the mesophyll cells. Statement II: Mesophyll cells of $C_4$ plants lack RuBisCo enzyme. In the light of the above statements, choose the correct answer from the options given below:

    • (A)
      Statement I is incorrect but Statement II is correct
    • (B)
      Both Statement I and Statement II are correct
    • (C)
      Both Statement I and Statement II are incorrect
    • (D)
      Statement I is correct but Statement II is incorrect
17. 17.

    NEET 2022

    What is the role of large bundle sheath cells found around the vascular bundles in $C_4$ plants?

    • (A)
      To protect the vascular tissue from high light intensity
    • (B)
      To provide the site for photorespiratory pathway
    • (C)
      To increase the number of chloroplasts for the operation of Calvin cycle
    • (D)
      To enable the plant to tolerate high temperature
18. 18.

    NEET 2021

    The first stable product of CO2 fixation in sorghum is :

    • (A)
      Succinic acid
    • (B)
      Pyruvic acid
    • (C)
      Phosphoglyceric acid
    • (D)
      Oxaloacetic acid
19. 19.

    NEET 2021

    Which of the following statements is incorrect ?

    • (A)
      Both ATP and NADPH+H⁺ are synthesized during non-cyclic photophosphorylation.
    • (B)
      Stroma lamellae have PS I only and lack NADP reductase.
    • (C)
      Grana lamellae have both PS I and PS II.
    • (D)
      Cyclic photophosphorylation involves both PS I and PS II.
20. 20.

    NEET 2020

    In light reaction, plastoquinone facilitates the transfer of electrons from :

    • (A)
      PS-II to $\mathrm{Cytb_6f}$ complex
    • (B)
      $\mathrm{Cytb_6f}$ complex to PS-I
    • (C)
      PS-I to $\mathrm{NADP^+}$
    • (D)
      PS-I to ATP synthase